| Registro completo |
| Provedor de dados: |
OAK
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| País: |
Japan
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| Título: |
Identification of ribosomal phosphoprotein P0 of Neospora caninum as a potential common vaccine candidate for the control of both neosporosis and toxoplasmosis
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| Autores: |
Zhang, Houshuang
Lee, Eung-goo
Liao, Min
Compaore, Muller K.A.
Zhang, Guohong
Kawase, Osamu
Fujisaki, Kozo
Sugimoto, Chihiro
Nishikawa, Yoshifumi
Xuan, Xuenan
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| Data: |
2007-06
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| Ano: |
2007
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| Palavras-chave: |
Neospora caninum
Toxoplasma gondii
Ribosomal phosphoprotein P0
Cross-reactive
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| Resumo: |
The characterization of the cross-reactive antigens of two closely related apicomplexan parasites, Neospora caninum and Toxoplasma gondii, is important to elucidate the common mechanisms of parasite–host interactions. In this context, a gene encoding N. caninum ribosomal phosphoprotein P0 (NcP0) was identified by immunoscreening of a N. caninum tachyzoite cDNA expression library with antisera from mice immunized with T. gondii tachyzoites. The NcP0 was encoded by a gene with open reading frame of 936 bp, which encoded a protein of 311 amino acids. The NcP0 gene existed as a single copy in the genome and was interrupted by a 432 bp intron. The NcP0 showed 94.5% amino acid identity to T. gondii P0 (TgP0). Anti-recombinant NcP0 (rNcP0) sera recognized a native parasite protein with a molecular mass of 34 kDa in Western blot analysis. Immunofluorescence analysis showed that the NcP0 was localized to the surface of N. caninum tachyzoites. A purified anti-rNcP0 IgG antibody inhibited the growth of N. caninum and T. gondii in vitro in a concentration-dependent manner. These results indicate that P0 is a cross-reactive antigen between N. caninum and T. gondii and a potential common vaccine candidate to control both parasites.
http://www.sciencedirect.com/science/journal/01666851
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| Idioma: |
Inglês
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| Identificador: |
http://ir.obihiro.ac.jp/dspace/handle/10322/1035
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| Editor: |
Elsevier
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| Formato: |
application/pdf
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